FIGURE

Fig. 4

ID

ZDB-FIG-171010-11

Publication

Bouldin et al., 2015 - Wnt signaling and tbx16 form a bistable switch to commit bipotential progenitors to mesoderm

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Fig. 4

tbx16 is Wnt responsive. (A-C) Immunofluorescence shows cells in the tailbud with nuclear β-catenin (nβ-catenin; A, orange; cytoplasmic and membrane-bound β-catenin have been eliminated from the image; see Materials and Methods) or Sox2 protein (B, orange). DAPI labels nuclei (blue). The highest levels of nβ-catenin and Sox2 are white and the lowest levels are purple. (C) A composite image shows that there is little overlap between the Sox2 domain (red) and the nuclear β-catenin domain (green). (D-G) Fluorescent whole-mount in situ hybridization shows tbx16 (red) and ntl (green) with HS:caβ-catenin (F,G) and in control (D,E) at 4 h post-heat shock. Single slices (D,F) and maximum intensity projections (E,G) are shown. In all images, anterior is to the left and posterior is to the right.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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