FIGURE

Fig. 5

ID

ZDB-FIG-171006-35

Publication

Du et al., 2014 - Setdb2 controls convergence and extension movements during zebrafish gastrulation by transcriptional regulation of dvr1

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Fig. 5

Identification of the essential role of Dvr1 in C&E cell movements using a cell-tracing strategy. (A–P) Tracking cell movements in C&E using UV-mediated photoactivation. At the one-cell stage, the embryos were injected with either 100 pg mRNA coding for Kaede protein plus dvr1 control morpholino or kaede mRNA plus dvr1/setdb2 morpholinos. Embryos were labeled by UV radiation at the shield stage and analyzed at the tail-bud stage. Dorsal mesendodermal cells in control (A,B, n=13), dvr1 mo^ATG (E,F, n=21), dvr1 mo^SB (I,J, n=10) and setdb2 mo (M,N, n=14). Lateral mesendodermal cells in control (C,D, n=13), dvr1 mo^ATG (G,H, n=22), dvr1 mo^SB (K,L, n=10) and setdb2 mo (O,P, n=18). (Q) Statistical analysis of anterior migration of labeled dorsal mesendodermal cells and dorsal migration of lateral mesendodermal cells. All images were lateral views. Asterisks indicate statistically significant differences (***, P<0.001; ****, P<0.0001). V, ventral side. D, dorsal side.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-setdb2 WT + MO2-gdf3 WT + MO5-gdf3
Knockdown Reagents:	MO1-setdb2 MO2-gdf3 MO5-gdf3
Observed In:	convergent extension involved in gastrulation
Stage Range:	Shield to Bud

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 392(2), Du, T.T., Xu, P.F., Dong, Z.W., Fan, H.B., Jin, Y., Dong, M., Chen, Y., Pan, W.J., Ren, R.B., Liu, T.X., Deng, M., Huang, Q.H., Setdb2 controls convergence and extension movements during zebrafish gastrulation by transcriptional regulation of dvr1, 233-44, Copyright (2014) with permission from Elsevier. Full text @ Dev. Biol.