FIGURE

Fig. 5

ID

ZDB-FIG-171002-5

Publication

Shimizu et al., 2017 - The Calcineurin-FoxO-MuRF1 signaling pathway regulates myofibril integrity in cardiomyocytes

Other Figures

All Figure Page

Back to All Figure Page

Fig. 5

Blocking MuRF1-mediated proteasome degradation preserves myofibril integrity in tre/ncx1 deficient hearts.

(A) Z-lines were visualized by α-actinin staining. By 72 hpf, sarcomeres are disassembled in hearts of uninjected (tre) and control morpholino-injected (tre +ctlMO) tremblor embryos. Murf1a/murf1 b knockdown does not affect sarcomere integrity in wild type embryos (WT +MO), but prevents sarcomere degradation in tre (tre +MO). Similarly, treatment with a proteasome inhibitor, MG132, preserves myofibril integrity in tre cardiomyocytes (tre +MG132). Scale bar, 10 μm. (B) Graph shows % of embryos with periodic α-actinin staining at 72 hpf. (C) Western blot detecting MuRF1 and β-actin proteins in uninjected control (WT control) and murf1a/murf1 b knockdown (MuRF1 MO) embryos. Chi-squared test, *p<0.05; **p<0.01; ***p<0.001.

Expression Data

Antibody:	Ab1-trim63
Fish:	WT WT + MO1-trim63a + MO1-trim63b
Knockdown Reagents:	MO1-trim63a MO1-trim63b
Anatomical Term:	whole organism
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	slc8a1a^{tc318d/tc318d} WT + MO1-trim63a + MO1-trim63b slc8a1a^{tc318d/tc318d} + MO1-trim63a + MO1-trim63b
Condition:	chemical treatment: proteasome inhibitor
Knockdown Reagents:	MO1-trim63a MO1-trim63b
Observed In:	cardiac muscle cell sarcomere cardiac muscle cell Z disc whole organism
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Elife