Fig. S1

Endogenous and BNF-induced CYP1 expression in other vascular beds.

A-D) High magnification images of cyp1a1/b1 expression in trunk vasculature of DMSO (A, B) and BNF (C, D) treated embryos. Arrowheads point to ISVs. cyp1a1 is expressed highly in PCV, ISVs and DLAV, and in isolated DA cells under normal conditions (A), and possible induction in blood vessels by BNF is obscured by high skin staining (C). No endogenous vascular cyp1b1 can be detected (B), but it is induced in DA, DLAV and ISVs by BNF (D). E) Camera lucida drawing of 48 hpf zebrafish embryo from Kimmel, et al 1995 [36]. Dashed lines indicate plane of images in this figure to visualize lateral dorsal aortae (LDA, green). Confocal image shows dorsal view of LDA in live embryo at 48 hpf (scale bar is 200 um). Cartoon schematic depicts arrangement of LDA and DA. F-M) Cranial expression of cyp1a1/b1 in DMSO-treated embryos. Expression of cyp1a1 is mostly vascular-specific and restricted to arteries (LDA, PICA and OA). (*) marks the liver. No vascular or liver expression of cyp1b1 is detected, and staining is only evident in the ear, middle of the brain and parts of the ventral eye. Arrow marks optic furrow. N-U) Cranial expression of cyp1a1/b1 in BNF-treated embryos. In addition to the basal expression domains, specific blood vessels upregulate cyp1b1 (the LDA and PICA). Note lack of cyp1b1 expression in the liver even under BNF stimulation (* in R). Embryos in N-Q are ahr2 mutants to enable imaging of interior vessels. Numbers of embryos analyzed are the same as in Fig 2. All scale bars are 100 um. Abbreviations–AHR: aryl hydrocarbon receptor, BNF: beta-naphthoflavone, CYP: cytochrome p450, DA: dorsal aorta, DLAV: dorsal longitudinal anastomotic vessel, DMSO: dimethylsulfoxide, hpf: hours post fertilization, ISV: intersegmental vessel, LDA: lateral dorsal aortae, OA: optic artery, PCV: posterior cardinal vein, PICA: primitive internal carotid artery.