FIGURE

Fig. 4

ID

ZDB-FIG-170921-99

Publication

Chernyavskaya et al., 2017 - Loss of DNA methylation in zebrafish embryos activates retrotransposons to trigger antiviral signaling

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Fig. 4

Leukocyte depletion does not reduce the induction of immune genes in uhrf1 mutants. Macrophages (A) and neutrophils (B) are enriched in the head (magnified in inset) of Tg(mpeg:mCherry;uhrf1) (A) and Tg(lysZ:dsRed;uhrf1) (B) and WT embryos at 80 and 120 hpf. Macrophages (C) and neutrophils (D) in the head region (inset in A,B) are significantly increased in uhrf1 mutants at 80 and 120 hpf. The number of embryos and clutches analyzed is indicated for each sample. (E) 0.5 mM pu.1 morpholino (MO) effectively reduces the leukocyte population. (F) RT-qPCR analysis reveals fold change in expression of immune genes from five clutches of uhrf1 and uhrf1 mutant/pu.1 morphants at 80 hpf (expression in WT embryos is shown in Fig. S5). A line connecting two dots represents one paired sample, which are siblings from a single clutch. P-values were determined by t-test (C,D) or paired t-test (F).

Expression Data

Genes:	DsRed2 mCherry
Fish:	gl23Tg nz50Tg gl23Tg + MO1-spi1b nz50Tg + MO1-spi1b uhrf1^{hi272Tg/hi272Tg}; gl23Tg uhrf1^{hi272Tg/hi272Tg}; nz50Tg uhrf1^{hi272Tg/hi272Tg}; gl23Tg + MO1-spi1b uhrf1^{hi272Tg/hi272Tg}; nz50Tg + MO1-spi1b
Knockdown Reagent:	MO1-spi1b
Anatomical Terms:	head macrophage head neutrophil
Stage Range:	Protruding-mouth to Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	gl23Tg + MO1-spi1b nz50Tg + MO1-spi1b uhrf1^{hi272Tg/hi272Tg}; gl23Tg uhrf1^{hi272Tg/hi272Tg}; nz50Tg uhrf1^{hi272Tg/hi272Tg}; gl23Tg + MO1-spi1b uhrf1^{hi272Tg/hi272Tg}; nz50Tg + MO1-spi1b
Knockdown Reagent:	MO1-spi1b
Observed In:	head macrophage head neutrophil macrophage
Stage Range:	Protruding-mouth to Day 5

Phenotype Detail

Acknowledgments

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