FIGURE

Fig. 4

ID

ZDB-FIG-170913-17

Publication

Kondrychyn et al., 2017 - Transcriptional Complexity and Distinct Expression Patterns of auts2 Paralogs in Danio rerio.

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Fig. 4

Expression of auts2b mRNA during zebrafish development. (A) RT-PCR analysis of auts2b expression during development (amplicon size is 3.25 kb). Numbers shown below each lane indicate sample age in hours post fertilization. (B–F'', I, I', and K–L'): Whole-mount in situ hybridization analysis of auts2b transcript expression in wild-type embryos at different developmental stages: 9 hr (B and B'), 10.3 hr (C and C'), 11 hr (D and D'), 12 hr (E–E''), 14 hr (F–F''), 18 hr (I and I'), 24 hr (K and K'), and 48 hr (L and L'). (G–H'', J, and J') Double in situ hybridization with egr2a (krox20) (G–G''), otx2 (H–H''), and sim1a (J and J’) as second probes. de, diencephalon; dth, dorsal thalamus; fb, forebrain; hb, hindbrain; mb, midbrain; mhb, midbrain-hindbrain boundary; r, rhombomere; rp, roof plate; RT-PCR, reverse transcription-polymerase chain reaction; s, somites; te, telencephalon; vde, ventral diencephalon; vth, ventral thalamus.

Expression Data

Genes:	auts2b sim1a
Fish:	WT
Anatomical Terms:	cerebellum diencephalon ventral side forebrain neural keel forebrain neural plate hindbrain hindbrain dorsal side hindbrain neural keel hindbrain neural rod midbrain neural keel presumptive diencephalon presumptive rhombomere 1 presumptive rhombomere 2 presumptive rhombomere 4 presumptive telencephalon rhombomere 1 rhombomere 2 rhombomere 4 roof plate roof plate spinal cord region somite spinal cord neural keel spinal cord neural rod telencephalon ventral thalamus whole organism
Stage Range:	1k-cell to Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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