FIGURE

Fig. 2

ID

ZDB-FIG-170911-6

Publication

Santos-Ledo et al., 2017 - Kinesin-1 promotes chondrocyte maintenance during skeletal morphogenesis

Other Figures

All Figure Page

Back to All Figure Page

Fig. 2

Loss of kif5B impairs chondrocyte secretion.

A-C) confocal images of Wt (A), single kif5Ba^ae12/ae12 mutants (B) and kif5Ba^ae12/ae12Kif5Bb^ae24/ae24 (C) at 60 hpf. Chondrocytes (Sox10:GFP positive cells) secrete proteins positive for peanut agglutinin (PNA, blue) homogeneously. Secretion in single (B) and compound (C) mutants is deficient. Stacking defects are evident in kif5Ba^ae12/ae12Kif5Bb^ae24/ae24 (C). D-F) Confocal images of Wt (D), single kif5Ba^ae12/ae12 mutants (E) and kif5Ba^ae12/ae12Kif5Bb^ae24/ae24 (F) at 5 dpf. In Wt (D) Di0C6 (blue) forms variably-sized cellular aggregates. Wheat Germ agglutinin (WGA, green) labels secreted proteins and II-II6B3 marks Collagen II (red) an abundant protein in cartilage. Both are homogeneously secreted in Wt and overlap within ECM (insets in D), although some overlap occurs between Collagen II and Di0C6 (insets in D). In single (E) and compound (F) mutants, Di0C6 forms large patches. Secretion is polarized with little colocalization within ECM (insets in E and F), large patches of Di0C6 colocalize with Collagen II, and cell stacking is perturbed (E, F). Scale bar: 50μm.

Expression Data

Gene:	EGFP
Antibody:	Ab1-col2a
Fish:	WT kif5ba^ae12/ae12 ba2Tg kif5ba^ae12/ae12; ba2Tg kif5ba^ae12/ae12; kif5bb^ae24/ae24 kif5ba^ae12/ae12; kif5bb^ae24/ae24; ba2Tg
Anatomical Terms:	chondrocyte extracellular matrix mandibular arch skeleton chondrocyte
Stage Range:	Pec-fin to Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	kif5ba^ae12/ae12 kif5ba^ae12/ae12; ba2Tg kif5ba^ae12/ae12; kif5bb^ae24/ae24 kif5ba^ae12/ae12; kif5bb^ae24/ae24; ba2Tg
Observed In:	chondrocyte endoplasmic reticulum chondrocyte extracellular matrix chondrocyte mitochondrion chondrocyte secretion mandibular arch skeleton chondrocyte
Stage Range:	Pec-fin to Day 5

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Genet.