FIGURE

Fig. 8

ID

ZDB-FIG-170907-37

Publication

Gerri et al., 2017 - Hif-1α regulates macrophage-endothelial interactions during blood vessel development in zebrafish

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Fig. 8

Most macrophages closely associated with unstable vessels are tnfa:EGFPF⁺.

(a) Schematic representation of the experiment shown in b–d. (b) Quantification at 66 hpf of tnfa:EGFPF⁺ and tnfa:EGFPF⁻ macrophages in normoxia and after DMOG treatment for 18 h starting at 48 hpf. (c) Maximal intensity projections of time-lapse confocal images of Tg(mpeg:mCherry);Tg(tnfa:EGFPF);Tg(kdrl:mCherry) WT embryo treated with DMOG starting at 48 hpf; lateral views. Yellow arrowhead points to an unstable vessel, white arrowheads point to recruited macrophages. (d) Quantification at 66 hpf of tnfa:EGFPF⁺ and tnfa:EGFPF⁻ macrophages associated with 15 unstable vessels after DMOG treatment starting at 48 hpf. 38 macrophages were observed, 23 tnfa:EGFPF⁺ and 15 tnfa:EGFPF⁻. Bars represent mean±s.e.m., n=15 embryos from three different clutches, (*P<0.05; NS, no significant changes observed; t-test). Scale bar, 50 μm.

Expression Data

Genes:	EGFP mCherry
Fish:	s896Tg; ump2Tg; ump5Tg
Condition:	chemical treatment by environment: dimethyloxalylglycine
Anatomical Terms:	dorsal longitudinal anastomotic vessel endothelial cell macrophage
Stage Range:	Long-pec to Pec-fin

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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