FIGURE

Fig. 3

ID

ZDB-FIG-170830-20

Publication

Xia et al., 2017 - Zebrafish slc30a10 deficiency revealed a novel compensatory mechanism of Atp2c1 in maintaining manganese homeostasis

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Fig. 3

Slc30a10 mutant zebrafish develop liver damage.

(A) Oil red O staining shows that mutant embryos have a slightly fatty liver (red arrows), which was worsened upon exposure to Mn. (B-C) HE staining (B) and Oil red O staining (C) of frozen sections showing hepatic steatosis in mutant embryos following Mn exposure. (D) Example images of a wild-type and mutant embryo under Mn exposure, showing a darker colored liver in the mutant (red arrow). (E) Dose-response curve showing the percentage of embryos with liver color change versus Mn concentration (n = 3 sets of 20 embryos/group). (F) Image of the liver of a wild-type and mutant adult, showing severe fibrosis in the mutant liver with sirius red staining. (G-H) The fibrosis markers col1a1a and ctgfa were measured in the liver of both male and female WT and mutant animals (n = 3 sets of 20 adults/group; *p<0.05 and **p<0.01).

Expression Data

Genes:	col1a1a ccn2a
Fish:	WT slc30a10^zju12/zju12
Condition:	chemical treatment by environment: manganese(II) chloride
Anatomical Term:	liver
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	slc30a10^zju12/zju12
Condition:	chemical treatment by environment: manganese(II) chloride
Observed In:	liver
Stage Range:	Day 6 to Adult

Phenotype Detail

Acknowledgments

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