FIGURE

Fig. 1

ID

ZDB-FIG-170807-33

Publication

Murphy et al., 2017 - Knockdown of epigenetic transcriptional co-regulator Brd2a disrupts apoptosis and proper formation of hindbrain and midbrain-hindbrain boundary (MHB) region in zebrafish

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Fig. 1

brd2aMO morphants exhibit reduced brain, ill-defined MHB, and trunk defects. Representative embryos from morpholino injection studies conducted on a total of at least three clutches and 40–60 embryos per treatment. A) uninjected, B) 4 ng MO1 five-base-mismatch-injected, C) 4 ng standard control MOc-injected, D) and E) 4 ng brdMO-injected, and F) 4 ng brd2aMO2-injected embryos, are compared at prim 5–6 stages. MO1 and MO2 target non-overlapping regions in brd2a mRNA. Control embryos appear equivalent to wildtype, while both MO1 and MO2 morphants show the same characteristic reduced brain and ill-defined midbrain-hindbrain boundary (MHB) (arrows in D,E,F).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + MO3-brd2a AB + MO4-brd2a
Knockdown Reagents:	MO3-brd2a MO4-brd2a
Observed In:	brain fourth ventricle midbrain hindbrain boundary post-vent region
Stage:	Prim-5

Phenotype Detail

Acknowledgments

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Reprinted from Mechanisms of Development, 146, Murphy, T., Melville, H., Fradkin, E., Bistany, G., Branigan, G., Olsen, K., Comstock, C.R., Hanby, H., Garbade, E., DiBenedetto, A.J., Knockdown of epigenetic transcriptional co-regulator Brd2a disrupts apoptosis and proper formation of hindbrain and midbrain-hindbrain boundary (MHB) region in zebrafish, 10-30, Copyright (2017) with permission from Elsevier. Full text @ Mech. Dev.