Fig. 3

The prps1a mutation causes a reduction in the eye, a loss of iridophores, and a reduction in neuromast hair cells.

(A,B) Eye phenotype in the control (A) and prps1a Zhom (B) embryos at 3 dpf. Black arrow in B points to the slightly smaller eye in the Zhom mutant. (A',B') Iridophore phenotype in control (A') and prps1a Zhom (B') embryos at 3 dpf. White arrows in B' point to the areas where iridophores are reduced in different tissues. (C,D) Quantification of the reduction in eye size (C) and iridophores (D). Eye area was calculated by image J. The numbers of iridophores were obtained by counting the iridophores in the dorsal and ventral side of the trunk area above the yolk extension. The reduction was significant in the eye size (n = 8, p < 0.001) and iridophores (n = 12, p < 0.001). (E–J) Immunohistochemical staining of phosphorylated histone H3 Ser10 in the control and MZhom embryos at 3 dpf. Columns from left to right show the representative images from the staining of phosphorylated histone H3 Ser10, DAPI, and the merged image. White arrows in H point to representative positive cells. (K) Quantification of phosphorylated histone H3 cells in the retina. The increased number of H3 Ser10 positive cells in the MZhom embryos is significant (n = 5 for Ctrl, n = 7 for MZhom, p < 0.001). (L–M) Fluorescent images of a wild-type Yopro-1 stained embryo at 3 dpf showing the location of neuromasts (L) and a higher magnification of the P1 neuromast (M). Hair cells in P1–P4 neuromasts were counted for prps1a mutants and control siblings. (N) prps1a homozygous mutants have a reduced number of neuromast hair cells at 3 dpf. The reduction is significant (n = 10, p < 0.001). Scale bars: 50 μm in (G,J); 10 μm in (M).