FIGURE

Fig. 3

ID

ZDB-FIG-170510-23

Publication

Chatzopoulou et al., 2017 - Functional analysis reveals no transcriptional role for the glucocorticoid receptor β-isoform in zebrafish

Other Figures

Fig. 3

All Figure Page

Back to All Figure Page

Fig. 3

Images of a 3dpf zebrafish embryo generated by crossing the Tg(hsp70:Gal4) and Tg(UAS:GFP-zGRβ) transgenic lines. Embryos were heat shocked at 37 °C for 2.5 h at 1 and 2dpf. A. Transmitted light microscopy image showing representative embryo, indicating that transgenesis and heat shock treatment do not alter the morphology of zebrafish embryos. B. Fluorescence microscopy image showing expression of the GFP-zGRβ fusion protein. The green fluorescent signal is clearly present in cells in the muscles and the eyes, and in cells lining the yolk sac. C. Larger magnification fluorescence microscopy image showing expression of GFP-zGRβ in muscle tissue. The image shows that GFP-zGRβ is localized in the nuclear cell compartment.

Expression Data

Gene:	d1EGFP
Fish:	ibl40Tg; kca4Tg
Condition:	heat shock
Anatomical Terms:	eye muscle nucleus yolk anatomical region
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Molecular and Cellular Endocrinology, 447, Chatzopoulou, A., Schoonheim, P.J., Torraca, V., Meijer, A.H., Spaink, H.P., Schaaf, M.J., Functional analysis reveals no transcriptional role for the glucocorticoid receptor β-isoform in zebrafish, 61-70, Copyright (2017) with permission from Elsevier. Full text @ Mol. Cell. Endocrinol.