FIGURE

Fig. 2

ID

ZDB-FIG-170425-28

Publication

Siegerist et al., 2017 - Acute podocyte injury is not a stimulus for podocytes to migrate along the glomerular basement membrane in zebrafish larvae

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Fig. 2

MTZ treatment leads to denudation of the GBM.

Panel a shows denudation of glomerular capillaries after 16 hours exposure to 5 mM MTZ of Chipper (Tg(fli1a:eGFP); Tg(nphs2:Eco.NfsB-mCherry); mitfa^w2/w2; roy^a9/a9) while control larvae showed uniform covering with mCherry positive podocytes (scale bar represents 10 μm). The phenotype in transmission electron micrographs of 4 dpf Cherry larvae after 24 hours MTZ treatment verified these findings (panel b), as broad areas of the GBM (arrowheads) were denuded, while control larvae showed normal morphology with foot processes and filtration slits. Endothelial cells of denuded areas showed significant fewer fenestrations (arrows). Note the higher contrasted Bowman’s space in 5 mM MTZ group compared to control caused by proteinaceous fluid due to leakiness of the glomerular filter (scale bar represents 1 μm, yellow = GBM, representative images from n = 3 experiments).

Expression Data

Genes:	EGFP mCherry
Fish:	mitfa^w2/w2; mpv17^a9/a9; mi1001Tg; y1Tg
Condition:	chemical treatment by environment: metronidazole
Anatomical Terms:	podocyte renal system capillary
Stage:	Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	mitfa^w2/w2; mpv17^a9/a9; mi1001Tg; y1Tg
Condition:	chemical treatment by environment: metronidazole
Observed In:	glomerular basement membrane glomerular capillary podocyte pronephric capsular space protein
Stage:	Day 4

Phenotype Detail

Acknowledgments

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