FIGURE

Fig. 6

ID

ZDB-FIG-170314-9

Publication

Williams et al., 2017 - Cyp1b1 Regulates Ocular Fissure Closure Through a Retinoic Acid-Independent Pathway

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Fig. 6

Tyr expression is not influenced by alterations in cyp1b1 expression. In situ hybridization demonstrated that at 24 hpf, tyr was expressed in the migrating neural crest and retinal pigment epithelium in uninjected (A) and control-injected (B) embryos. At 48 hpf, tyr expression (A', A'', B', B'') followed the skin pigmentation pattern and was also observed in the cranial neural crest (arrowheads), retinal pigment epithelium, migrating neural crest cells in the ocular fissure (closed arrows), and iris stroma (open arrows). Knockdown of cyp1b1 did not alter tyr expression at 24 hpf, but did decrease tyr expression in the cranial neural crest (arrowhead) and ocular fissure (C, C') at 48 hpf. Overexpression of cyp1b1 did not alter tyr expression (D, D', D'') at 24 and 48 hpf.

Expression Data

Gene:	tyr
Fish:	WT WT + MO3-cyp1b1
Knockdown Reagent:	MO3-cyp1b1
Anatomical Terms:	eye iris stroma migratory neural crest neural crest presumptive retinal pigmented epithelium retinal pigmented epithelium
Stage Range:	Prim-5 to Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO3-cyp1b1
Knockdown Reagent:	MO3-cyp1b1
Observed In:	eye migratory neural crest
Stage:	Long-pec

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Invest. Ophthalmol. Vis. Sci.