FIGURE

Fig. 1

ID

ZDB-FIG-170308-14

Publication

Madelaine et al., 2017 - The hypothalamic NPVF circuit modulates ventral raphe activity during nociception

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Fig. 1

NPVF circuit interacts with the raphe nucleus.

(A) Alignment of human, mouse and zebrafish NPVF precursors. Multiple sequence alignments show strong conservation in the sequence of putative NPSF (blue) and NPVF (red) neuropeptides. Stars indicate sequence identity across the alignment. (B) Schematic of larval zebrafish, with location of NPVF neurons (hyp.: hypothalamus; vRN: ventral raphe nucleus). (C) Confocal Z-projection of Tg(npvf:EGFP) larval fish at 5 dpf. Hypothalamus and vental raphe nucleus are highlighted in Box 1 and 2 respectively. (D) Confocal overlay of EGFP+ neurons and NPVF peptide in the hypothalamus at 5 dpf. (E) eGFP+ axons and 5HT antibody stain in the raphe nucleus at 5 dpf. (F) Neurons in the vRN are positive for in situ hybridization for gpr147 at 5 dpf. Scale bars: C = 100 μm, D, E and F = 10 μm.

Expression Data

Genes:	EGFP npffr1
Antibody:	Ab1-npvf
Fish:	zf775Tg
Anatomical Terms:	hypothalamus raphe nucleus
Stage:	Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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