FIGURE

Fig. 2

ID

ZDB-FIG-170222-33

Publication

Heanue et al., 2016 - A Novel Zebrafish ret Heterozygous Model of Hirschsprung Disease Identifies a Functional Role for mapk10 as a Modifier of Enteric Nervous System Phenotype Severity

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Fig. 2

ret^hu2846/+ larvae exhibit ENS progenitor migration deficits.

ENS progenitor migration at the front of migration (wavefront) visualized in real time by virtue of the SAGFF234A;UAS:GFP background labeling ENCCs. (A) Representative still images from confocal time-lapse recordings (depth-coded to resolve 2 migratory streams) of WT (ret^+/+) and ret^hu2846/+ embryos at 0 minutes (48hpf) and 500 minutes (56hpf), show that distance travelled by the migration wavefront is decreased in ret^hu2846/+ larvae relative to WT larvae. Asterisk denotes a GFP+ cell from outside the ENS linage that remains in the same position. (B) The calculated wavefront migration speed (microns/minute) is significantly reduced in ret^hu2846/+ larvae relative to WT at 48–56hpf (p = 0.0022), and 72–80hpf (p = 0.0182).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	ret^hu2846/+; nksagff234aGt; nkuasgfp1aTg
Observed In:	enteric nervous system development neural crest cell migration involved in autonomic nervous system development
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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