FIGURE

Fig. 2

ID

ZDB-FIG-170222-22

Publication

Fontenas et al., 2016 - Neuronal Ndrg4 Is Essential for Nodes of Ranvier Organization in Zebrafish

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Fig. 2

ndrg4 is required for sodium channel and neurofascin clustering in the peripheral nervous system.

(A-C) Lateral view of mbp RNA expression in control (A), ndrg4 mutant (B) and morphant (C) embryos at 4 dpf. Arrows indicate mbp-expressing cells along the PLLn. Scale bars = 200μm. (D-L) Acetylated tubulin (ac tub; red) and sodium channels (NaCh; green) immunohistochemistry of a (D-F) control, ndrg4 mutant (G-I) and ndrg4 morphant (J-L) PLLn at 4 dpf. Scale bars = 5μm. (M) High magnification of three nodes of Ranvier (arrowheads) from a control nerve. Scale bar = 100nm. (N) A significant decrease in the number of sodium channels clusters is observed in ndrg4 mutants and morphants in comparison to controls (p<0.001). Acetylated tubulin (ac tub; red) and FIGQY (green) immunohistochemistry of a (D’-F’) control, ndrg4 mutant (G’-I’) and ndrg4 morphant (J’-L’) PLLn at 4dpf. Scale bars = 5 μm. (O,P) A significant decrease in the number of FIGQY labeled clusters is observed in ndrg4 mutants and morphants in comparison to controls (p<0.001).

Expression Data

Gene:	mbpa
Antibodies:	Ab1-nfasc Ab1-scn
Fish:	WT WT + CRISPR1-ndrg4 WT + MO2-ndrg4
Knockdown Reagents:	CRISPR1-ndrg4 MO2-ndrg4
Anatomical Terms:	posterior lateral line nerve anatomical region posterior lateral line nerve sodium channel complex
Stage:	Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + CRISPR1-ndrg4 WT + MO2-ndrg4
Knockdown Reagents:	CRISPR1-ndrg4 MO2-ndrg4
Observed In:	posterior lateral line nerve node of Ranvier
Stage:	Day 4

Phenotype Detail

Acknowledgments

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