FIGURE

Fig. 6

ID

ZDB-FIG-170208-32

Publication

Hu et al., 2016 - Liver-Enriched Gene 1, a Glycosylated Secretory Protein, Binds to FGFR and Mediates an Anti-stress Pathway to Protect Liver Development in Zebrafish

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Fig. 6

N⁷⁰-glycosylation is necessary for the secretion of Leg1b but not Leg1a.

(A) Western blot analysis of secretion of Leg1a, Leg1a^N70A, Leg1b and Leg1b^N70A. Mutating N⁷⁰ to A⁷⁰ does not affect the secretion of Leg1a^N70A but does affect Leg1b^N70A. Total protein was extracted from cell pellets or supernatant. HA-tagged RNaseI was used as a control. Western blot was repeated three times.(B) Co-immunostaining of Leg1a or Leg1a^N70A with the cis- and medial-Golgi marker Giantin in HepG2 cells transfected with WT leg1a and leg1a^N70A plasmids, respectively. (C) Co-immunostaining of Leg1b or Leg1b^N70Awith Giantin showed that Leg1b is secreted (upper panels) but Leg1b^N70A is retained in the cis-Golgi network (lower panels). (D) Co-immunostaining of Leg1b or Leg1b^N70A with the ER marker PDI in HepG2 cells transfected with WT leg1b and leg1b^N70A plasmids, respectively. The enlarged view of the area highlighted with dashed lines are shown on the right.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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