FIGURE

Fig. S5

ID

ZDB-FIG-170201-32

Publication

Rydeen et al., 2016 - Cyp26 Enzymes Facilitate Second Heart Field Progenitor Addition and Maintenance of Ventricular Integrity

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Fig. S5

SHF progenitors are specified in Cyp26-deficient embryos.

(A-F) ISH of mef2cb at 16 somites (s), 24, and 30 hpf in control and Cyp26-deficient embryos. (G,H) ISH of ltbp3 at 30 hpf in control and Cyp26-deficient embryos. Arrows in C-H indicate expression in the OFT. (I) RT-qPCR for mef2cb at 16 s, 24, 36 and 48 hpf. (J) RT-qPCR for ltbp3 at 16 s, 24, 36 and 48 hpf. (K) RT-qPCR for nkx2.5 at 16 s, 24, 36 and 48 hpf. (L) Graph depicting total number of nkx2.5+ cells at 24 hpf (n = 11 for control, n = 14 for Cyp26-deficient). (M) Graph depicting the percentage of proliferating nkx2.5+ cells at 24 hpf (n = 11 for control, n = 14 for Cyp26-deficient). (N) RT-qPCR for tbx1 at 16 s, 24, 36, and 48 hpf. For RT-qPCR, fold difference of mRNA was calculated relative to β-actin. (O-R) ISH for tbx1 at 24 and 30 hpf in control and Cyp26-deficient embryos. Arrows indicate tbx1 staining adjacent to the heart. Error bars are SEM, asterisks denote p<0.05 by Student’s t-test. Dorsal view, anterior up (A-D), lateral view anterior right (E-H); n>20 embryos per group for (A-H). Scale bars: 50 mm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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