FIGURE

Fig. 4

ID

ZDB-FIG-170130-17

Publication

Müller-Deile et al., 2016 - Overexpression of TGF-β Inducible microRNA-143 in Zebrafish Leads to Impairment of the Glomerular Filtration Barrier by Targeting Proteoglycans

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Fig. 4

Knockdown of syndecan 2 and versican causes edema and proteinuria in zebrafish larvae. (A) Edema quantification of zebrafish larvae at 120 hpf. Zebrafish were injected with a specific morpholinos for syndecan 3 (sdc3-MO, 100 μM), syndecan 4 (sdc4-MO, 100 μM), versican (vcan-MO, 100 μM), a scrambled control (CTRL-MO, 100 μM) or a combination of syndecan 3 (sdc3- MO, 100 μM) and syndecan 4 (sdc4-MO, 100 μM) at the one to four cell stage as indicated. The edema phenotypes of the larvae were categorized into 4 groups: P1 = no edema, P2 = mild edema, P3 = severe edema, P4 = very severe edema. (B) Corresponding fluorescent images of the retinal vessel plexus of Tg(lfabp: DBP:EGFP) of zebrafish larvae expressing a high molecular weight fluorescent protein in the circulation. Scatter graph presents maximum of circulating fluorescence intensity in the fish eye at 120 hpf, analyzed with image J. *** p<0.001, n.s. not significant; hpf: hours post fertilization.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	lri500Tg + MO1-vcana lri500Tg + MO2-sdc3 lri500Tg + MO4-sdc4 lri500Tg + MO2-sdc3 + MO4-sdc4
Knockdown Reagents:	MO1-vcana MO2-sdc3 MO4-sdc4
Observed In:	blood protein pericardium
Stage:	Day 5

Phenotype Detail

Acknowledgments

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