FIGURE

Fig. S2

ID

ZDB-FIG-170123-34

Publication

Drummond et al., 2017 - The tbx2a/b transcription factors direct pronephros segmentation and corpuscle of Stannius formation in zebrafish

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Fig. S2

tbx2a/b splice MO efficiency verification using RT-PCR. (A) Whole mount in situ hybridization reveals that tbx2a knockdown by tbx2a splice-blocking MO causes a decrease in the DL segment, marked by slc12a3. The tbx2a splice MO targets the splice acceptor site of intron 1, causing a fusion of exon 1 and exon 3. This change can be resolved by gel electrophoresis of RT-PCR wild-type and tbx2a splice morphant products. Sequencing revealed that the lower band present in morphants (asterisk) is the excised exon 2. (B) Similarly to effects of the tbx2a splice-blocking MO, the tbx2b splice-blocking MO causes a decrease in the DL segment. The tbx2b splice MO was designed to block the exon 3 splice donor site, resulting in an excision of exon 3. Sequencing revealed that the lower band present only in tbx2b splice morphant RT-PCR product is exon 3 from the tbx2b gene.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 421(1), Drummond, B.E., Li, Y., Marra, A.N., Cheng, C.N., Wingert, R.A., The tbx2a/b transcription factors direct pronephros segmentation and corpuscle of Stannius formation in zebrafish, 52-66, Copyright (2017) with permission from Elsevier. Full text @ Dev. Biol.