FIGURE

Fig. 1

ID

ZDB-FIG-170118-7

Publication

Pinzon-Olejua et al., 2017 - Cre inducible site-specific recombination in zebrafish oligodendrocytes

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Fig. 1

mCherry expression in 4- and 6-dpf Tg(mbpa:mCherry-T2A-CreER^T2) larvae. A: Scheme of the Tg(mbpa:mCherry-T2A-CreERT^T2) construct, which expresses a single open reading frame coding for mCherry (red) and CreER^T2 (dark gray) separated by a viral T2A peptide sequence (white) under the control of the Zebrafish mbpa promoter (light gray). B–I: Native mCherry fluorescence in 4-dpf (B–E) and 6-dpf (F–I) transgenic Zebrafish larvae. B,F: Lateral view of the larvae, showing mCherry expression in presumptive myelinating cells. Scale bar, 200 µm. C,D,G,H: Higher magnification of the areas indicated by white boxes in B,F. C,G: mCherry expressing oligodendrocytes in the hindbrain and (D,H) the spinal cord. E,I: Dorsal view of the hindbrain with mCherry fluorescence in presumptive oligodendrocyte cell bodies (arrowhead) and processes (arrow). Asterisks mark auto fluorescence from yolk and pigmentation. Scale bar, 100 µm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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