Fig. 2 S2

Comparable proliferation in the IM of wild-type and han^s6 mutant embryos.

(A, B) Immunofluorescence for Pax2a and phospho-Histone H3 (pH3) in wild-type (A) and han^s6 mutant (B) embryos at the 6 somite stage; dorsal views, anterior to the left, are three-dimensional reconstructions of 400 µm long regions used for quantification of the numbers of Pax2a⁺ (A', B') and pH3⁺ (A'', B'') cells. Arrows indicate Pax2a⁺ pH3⁺ cells. Scale bar represents 50 μm. (C) Table compares the number of Pax2a⁺ pH3⁺ cells per 100 µm of IM, the number of Pax2a⁺ cells per 100 µm of IM, and the proliferation index (Pax2a⁺ pH3⁺ cells / Pax2a⁺ cells X 100) in wild-type and han^s6 mutant embryos. Data shown represent the average and standard deviation for each value. While there was a statistically significant increase in the number of Pax2a⁺ cells in han^s6 mutants compared to wild-type (p<0.0001), no significant differences were observed for the number of Pax2a⁺ pH3⁺ cells (p=0.1461) or the proliferation index (p=0.0654).