Fig. S3

Epistatic analysis of wdr68 and edn1.

A-B) ISH analysis for edn1 expression on 25-somites stage animals raised at 28.5C that were heat shocked at the bud and 20-somites stages. A) wildtype sibling with normal edn1 expression. B) Tg(hsp70l:GFP-wdr68) embryo overexpressing GFP-Wdr68 with near-normal edn1 expression. C-D) ISH analysis on prim-12 stage embryos. C) wildtype control sibling with normal dlx6a expression. D) Et1-MO;wdr68^{hi3812/hi3812} mutant lacking dlx6a expression in all arches. E-F) merged green-red channel fluorescence on prim-5 stage embryos injected with either GFP/dsRed (G/R) or GFP/Edn1 (G/Edn1) plasmid mixtures. E) broad GFP/dsRed expression in a G/R embryo. F) broad GFP expression in a G/Edn1 embryo. G-H) lateral view of 5dpf alcian blue stained embryo. G) Et1-MO injected animal showing loss of M and CH but retention of PQ. H) Et1-MO;wdr68^{hi3812/hi3812} mutant showing loss of M, CH, and PQ. I-P) ISH analysis for hand2 expression on prim-12 stage animals raised at 32°C. Red arrowhead points at 1^st arch expression of hand2. I, K, M, O) dorsal view. J, L, N, P) lateral view. I, J) wildtype sibling injected with G/R mix showing normal hand2. K, L) wdr68^{hi3812/hi3812} mutant injected with G/R mix showing loss of 1^st arch hand2. M, N) wildtype sibling injected with G/Edn1 mix showing normal hand2. O,P) wdr68^{hi3812/hi3812} mutant injected with G/Edn1 mix showing loss of 1^st arch hand2.