FIGURE

Fig. 5

ID

ZDB-FIG-161206-20

Publication

Zada et al., 2016 - Pharmacological and BBB-targeted genetic therapies for thyroid hormone-dependent hypomyelination

Other Figures

All Figure Page

Back to All Figure Page

Fig. 5

Specific expression of Mct8 in the vascular system and BBB rescues the number of oligodendrocytes in the brain. (A,B) Lateral view of the trunk (A, head pointing to the right) and dorsal view of the head (B) of a tg(fli:Mct8-EGFP) 3-dpf embryo. (C-F) Lateral view of the trunk (C,E, head pointing to the right) and dorsal view of the head (D,F) of a tg(mbp:EGFP) larvae injected with fli:Mct8-tagRFP. Arrows indicate the expression of Mct8-tagRFP in the endothelial cells of the vascular system, and arrowheads indicate oligodendrocytes in the CNS. Wide (C) and high-magnification (dashed box in C, E) views in 3-dpf larvae show red mosaic expression of Mct8-tagRFP and green oligodendrocytes. Dorsal view of the head shows green oligodendrocytes in the brain and mosaic expression of Mct8-tagRFP in 3 (D)- and 10 (F)-dpf larvae. (G) Number of oligodendrocytes counted in the spinal cord (SC) of 3-dpf larvae (mct8^+/+: n=37, mct8^−/−: n=42 and mct8^−/−/Mct8-tagRFP: n=26, **P<0.01). (H) Number of oligodendrocytes counted in the brain of 10-dpf larvae (mct8^+/+: n=10, mct8^−/−: n=10 and mct8^−/−/Mct8-tagRFP: n=11, **P<0.01). Values represented as means±s.e.m. Statistical significance determined by one-way ANOVA followed by post-hoc Tukey HSD test (**P<0.01). Scale bars: 50 µm.

Expression Data

Gene:	EGFP
Fish:	biu10Tg
Anatomical Term:	endothelial cell
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	slc16a2^biu4/biu4; ck1Tg
Observed In:	brain oligodendrocyte spinal cord oligodendrocyte
Stage Range:	Protruding-mouth to Days 7-13

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Dis. Model. Mech.