FIGURE

Fig. 4

ID

ZDB-FIG-161201-16

Publication

Shin et al., 2016 - Vegfc acts through ERK to induce sprouting and differentiation of trunk lymphatic progenitors

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Fig. 4

ERK signaling is essential for trunk lymphatic morphogenesis and differentiation. (A-I) Confocal images; lateral views, anterior to the left, dorsal is up. (A,B) pERK (red) and GFP (green) immunostaining in Tg(fli1a:egfp)^y1 embryos of the indicated genotype at 34 hpf. Arrows denote pERK-positive lympho-venous sprouts, arrowheads indicate pERK staining in motor neurons. Brackets indicate DA and PCV. (C) Prox1 (red) and pERK (green) immunostaining in a wild-type embryo at 38 hpf. Arrows denote endothelial cells that are positive for Prox1 and pERK in lymphatic sprouts or dorsal wall of PCV. (D-I) Embryos treated with DMSO (D-F) or 15 µM SL327 (G-I). Treatment time is indicated. (D,G) Tie2 immunostaining; arrows denote lympho-venous sprouts, PCV indicated by bracket. (E,H) Prox1 (red) and GFP (green) immunostaining of Tg(fli1a:egfp)^y1 embryos at 36 hpf; arrows denote Prox1-positive lymphatic sprouts, arrowheads indicate muscle pioneers and Corpuscle of Stannius (cs) is indicated. (F,I) Live Tg(fli1a:egfp)^y1 embryos at 49 hpf. Arrows indicate parachordal cells, or absence thereof.

Expression Data

Gene:	EGFP
Antibodies:	Ab2-tek Ab9-mapk Ab10-prox1
Fish:	WT y1Tg flt4^um200/um200; y1Tg
Condition:	chemical treatment by environment: SL-327
Anatomical Terms:	corpuscles of Stannius dorsal aorta endothelial cell lymphangiogenic sprout motor neuron muscle pioneer posterior cardinal vein posterior cardinal vein endothelial cell posterior cardinal vein vascular sprouts vascular sprouts
Stage Range:	Prim-15 to Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT y1Tg flt4^um200/um200; y1Tg
Condition:	chemical treatment by environment: SL-327
Observed In:	lymphangiogenic sprout parachordal vessel parachordal vessel vasculature development posterior cardinal vein endothelial cell posterior cardinal vein vascular sprouts posterior caudal vein vascular sprouts vascular sprouts
Stage Range:	Prim-15 to Long-pec

Phenotype Detail

Acknowledgments

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