FIGURE

Fig. 1

ID

ZDB-FIG-161028-21

Publication

Warga et al., 2016 - Progressive loss of RacGAP1/ogre activity has sequential effects on cytokinesis and zebrafish development

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Fig. 1

The ogre mutant phenotype. (A and B) Live embryos; (C) after acridine orange staining to show cell death; and (D) after DAPI-squash to show nuclei. (E-H) anti-Active Caspase 3 staining showing progression of apoptosis; and with dlA expression (I) marking neural tissue in the brain and spinal cord, with myoD expression (K) marking somite muscle in the trunk, and with gata1a expression (M) marking blood in the posterior blood island. (J, L, N) Are high magnification cross-sections through the areas indicated with lines and tissues are circled while arrows indicate apoptotic cells; abbreviations: nt, neural tissue; nc, notochord. (O-Q) Expression of: (O) tal1 mRNA marking blood cells (arrows) and (P) Acetylated Tubulin marking trigeminal (trg) and Rohon-Beard sensory (rb) neurons (arrows). (Q) High magnification of mutant axons, showing abnormal varicosities (arrows).

Expression Data

Gene:	tal1
Antibodies:	Ab1-casp3 Ab1-tuba
Fish:	WT racgap1^ta52a/ta52a
Anatomical Terms:	head intermediate cell mass of mesoderm sensory neuron axon tail bud trunk
Stage Range:	1-4 somites to Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	racgap1^ta52a/ta52a
Observed In:	apoptotic process blastoderm apoptotic process blood cell cell embryonic structure nucleus head intermediate cell mass of mesoderm optic primordium sensory neuron axon tail bud trunk whole organism whole organism anterior-posterior axis
Stage Range:	Sphere to Prim-5

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 418(2), Warga, R.M., Wicklund, A., Richards, S.E., Kane, D.A., Progressive loss of RacGAP1/ogre activity has sequential effects on cytokinesis and zebrafish development, 307-22, Copyright (2016) with permission from Elsevier. Full text @ Dev. Biol.