FIGURE

Fig. 5

ID

ZDB-FIG-160927-51

Publication

Zhao et al., 2016 - Enhanced angiogenesis, hypoxia and neutrophil recruitment during Myc-induced liver tumorigenesis in zebrafish

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Fig. 5

Stimulation of tumorigenic liver growth by tumor-infiltrated neutrophils.

TO(Myc) and Tg(mpx:EGFP) double transgenic larvae were used for norpholino knockdown of Gcsfr to inhibit neutrophil differentiation. Morpholino oligonucleotides were injected into the embryos at 1-2-cell stage. (A-D) Images of the double transgenic larvae after injection of either MO(control) (A,C) or MO(gcsfr) (B,D). These injected embryos were either treated with Dox (C,D) or without Dox from 4 dpf to 7 dpf (A,B). The liver areas are circled. The original magnification was 20x. (E) Neutrophil counts in the liver. (F) Neutrophil density in the liver under different conditions. (G) Quantification of 2D liver size. The quantitative data were based on 5 samples per concentration group. Data are represented as mean ± SD. Asterisks indicate significant difference with P-value < 0.05 by unpaired t-test statistical analysis. Scale bars = 100 µm.

Expression Data

Gene:	EGFP
Fish:	gz26Tg; rj30Tg + MO3-csf3r
Condition:	chemical treatment by environment: doxycycline
Knockdown Reagent:	MO3-csf3r
Anatomical Term:	neutrophil
Stage:	Days 7-13

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	gz26Tg; rj30Tg gz26Tg; rj30Tg + MO3-csf3r
Condition:	chemical treatment by environment: doxycycline
Knockdown Reagent:	MO3-csf3r
Observed In:	liver liver neutrophil
Stage:	Days 7-13

Phenotype Detail

Acknowledgments

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