FIGURE

Fig. 8

ID

ZDB-FIG-160927-26

Publication

Jim et al., 2016 - Infection of zebrafish embryos with live fluorescent Streptococcus pneumoniae as a real-time pneumococcal meningitis model

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Fig. 8

Time-lapse fluorescence imaging of Streptococcus pneumoniae-infected zebrafish embryos via the hindbrain ventricle at 2 days post fertilization. a-c Live fluorescence images of double-labelled Tg(mpx:GFP) ⁱ¹¹⁴ /Tg (mpeg1:mCherry) ^gl23 zebrafish embryos (green fluorescent neutrophils, red fluorescent macrophages). Dorsal view of the head region after injection with a PBS or b, c S. pneumoniae D39 with the corresponding Z-position (I) and bright-field image (II). Images b and c were acquired from the same zebrafish embryo at different positions. b (III) After injection of pneumococci in the hindbrain ventricle, green fluorescent neutrophils migrate in increasing numbers to the site of infection compared to a (III) PBS-injected zebrafish embryos. Macrophages were not observed in the subarachnoid space during b early pneumococcal infection of the hindbrain ventricle but c (III) remain localized in regions below the subarachnoid space. Scale bars, 100 µm

Expression Data

Genes:	GFP mCherry
Fish:	gl23Tg; i114Tg
Condition:	bacterial treatment by injection: Streptococcus pneumoniae
Anatomical Terms:	macrophage neutrophil
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	gl23Tg; i114Tg
Condition:	bacterial treatment by injection: Streptococcus pneumoniae
Observed In:	brain neutrophil
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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