FIGURE

Fig. 10

ID

ZDB-FIG-160927-18

Publication

Robra et al., 2016 - The Intracellular Signaling Molecule Darpp-32 Is a Marker for Principal Neurons in the Cerebellum and Cerebellum-Like Circuits of Zebrafish

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Fig. 10

Darpp-32 expression in the larval optic tectum. Single optical sections of whole mount immunofluorescence stainings. The images are showing one hemisphere of the optic tectum as indicated in the schematic on the right side. (A) Darpp-32 expression in the optic tectum can be first seen at 3 dpf. Darpp-32 is expressed in the neuropil and the cell bodies of projection neurons. (B) Darpp-32 expression in optic tectum at 6 dpf. (C-E) Note that during the course of development the expression of Pvalb7 (D) and Darpp-32 (C) does not overlap in the optic tectum (E). In (C) intense Darpp-32 expression is visible in the superficial most layer, presumably the SM, while Pvalb 7 expression is detectable more closer to the midline, presumably in neurons of the stratum periventriculare (SPV). Darpp-32 positive cell bodies located are more lateral compared to Pvalb 7 positive ones. Scale bar in (A) 20 µm; in (B-E) 50 µm.

Expression Data

Gene:	ppp1r1b
Antibodies:	Ab1-ppp1r1b Ab1-pvalb
Fish:	WT
Anatomical Term:	optic tectum
Stage Range:	Protruding-mouth to Days 7-13

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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