Fig. 2
Depleting phagocytes by injection of clodronate liposomes does not affect CM proliferation. (a,b) The impact of control and clodronate liposome injection on the presence of leucocytes was tested by labelling the hearts of mpeg1:GFP transgenic fish (green) with L-plastin (red). (c,d) CM proliferation was quantified by measuring the number of CMs co-expressing DsRed2-nuc (red) and MCM5 (green). Double-positive cells are indicated with arrows. (e,f) Quantification of L-plastin- and mpeg1:GFP-positive area. (g,h) Quantification of proliferative CM and non-CM nuclei. (n ≥ 4 hearts; ≥2 sections per heart; ***p < 0.001; n.s., non-significant). |