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Fig. 1

ID
ZDB-FIG-160725-8
Publication
Kuipers et al., 2016 - Large-scale Scanning Transmission Electron Microscopy (Nanotomy) of Healthy and Injured Zebrafish Brain
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Fig. 1

Workflow of Large-scale Tissue Electron Microscopy on Zebrafish Brains. (A) 7 day-old zebrafish larva. (B) Zebrafish larval heads embedded side by side in epoxy resin. (C) Glass knife for semi -thin sectioning and orienting the specimen. (D) Representative semithin toluidine blue stained section showing two side-by-side embedded zebrafish larvae. (E) Diamond knife to cut ultrathin (70 nm) sections. (F) Edited image in (D) of whole zebrafish larval brain section on one-hole grid to indicate positioning. (G) Microscopy system used for large scale 2D EM in this study. (H) Flow of image processing.

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Antibody Labeling
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