FIGURE

Fig. 7

ID

ZDB-FIG-160708-9

Publication

He et al., 2016 - Synergy between loss of NF1 and overexpression of MYCN in neuroblastoma is mediated by the GAP-related domain

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Fig. 7

GRD domain is required for the tumor suppressor function of nf1 in MYCN-driven neuroblastoma tumorigenesis.

(A) Tumor penetrance of stable transgenic zebrafish with the genotypes of nf1a-/-;nf1b+/+;GFP;wt-GRD;mCherry (n = 31), nf1a-/-;nf1b+/+;GFP;mut-GRD;mCherry (n = 20) and nf1a-/-;nf1b+/+;GFP;mCherry (n = 41) at the age of 12 weeks. (B) nf1a-/-;nf1b+/+;MYCN;GFP fish injected with dbh:wt-GRD;dbh:mCherry (n = 58) showed a significantly lower mCherry+ mosaic tumor rate compared with the sibling fish injected with dbh:mut-GRD;dbh:mCherry (n = 25) at the age of 7 weeks (Fisher’s exact test), indicating the tumor suppression function of the NF1 GRD domain.Most early tumors arose in nf1a-/-;nf1b+/+;MYCN;GFP fish injected with dbh:wt-GRD; dbh:mCherry only expressed GFP (C) but not mCherry fluorescent protein (D). A significant subset of early tumors arose in nf1a-/-;nf1b+/+;MYCN;GFP fish injected with dbh:mut-GRD; dbh:mCherry did express both GFP (E) and mCherry (F).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	nf1a^p301/p301; zdf16Tg nf1a^p301/p301; zdf16Tg; zdf23Tg nf1a^p301/p301; zdf16Tg; zdf24Tg
Observed In:	interrenal gland neuroblastoma
Stage:	Adult

Phenotype Detail

Acknowledgments

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