FIGURE

Fig. S3

ID

ZDB-FIG-160609-23

Publication

Steed et al., 2016 - klf2a couples mechanotransduction and zebrafish valve morphogenesis through fibronectin synthesis

Other Figures

All Figure Page

Back to All Figure Page

Fig. S3

Defects in multi-layering occur in the presence of abnormal flow.

Cells in the superior AVC of Tg(fli:kaede) embryos injected as shown were photoconverted in the superior AVC (schematic shown) at 48hpf and allowed to develop until 80hpf in all panels. (A) In tnnt2aMO-injected hearts, lacking heart contraction, multi-layering failed to occur by 80hpf (n=3 control, n=3 tnnt2aMO). Boxed red areas in (A) are shown in (B). Photoconverted cells remained in the single-layered endocardium. White dashed line highlights the photoconverted cells of the superior AVC. (C) Many cells were observed in the cardiac jelly of gata1MO-injected hearts (yellow dashed line highlights cells in the cardiac jelly) and intermingling of non-photoconverted (green) and photoconverted (magenta) cells was sometimes observed (n=3/6) (D). Boxed red areas from (C) are shown in (D). In contrast, multi-layering was impaired in gata2MO-injected and failed to occur in myh6 MOinjected embryos. n=3 control, n=6 gata1MO, n=7 gata2MO, n=8 myh6 MO. Green dashed line highlights the non-photoconverted cells of the superior AVC. (E and F) Quantification of GFP signal intensity in klf2a:H2B-eGFP embryos demonstrates reduced klf2a expression in myh6 MO hearts (n=4 control hearts, n=3 myh6MO hearts). Error bar represents the standard deviation. Scale bars: 10 µm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Nat. Commun.