FIGURE

Fig. 5

ID

ZDB-FIG-160428-13

Publication

Sallin et al., 2016 - Acute stress is detrimental to heart regeneration in zebrafish

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Fig. 5

RNA-seq analyses of control versus stressed animals identified three candidate genes, nr4a1, ankrd9 and igfbp1b, which are expressed in non-CMs of cryoinjured hearts. (a,d,g) Sections of hearts at 14 dpci immunostained against tropomyosin (TPM, green) and DAPI (blue). (b,e,h) The same sections as in (a,d,g) after in situ hybridization with RNA antisense probes. (c,f,i) Higher magnification of the framed area shown in (b,e,h) overlapped with tropomyosin (TPM, c′,f′,i′) immunostaining. (j,k) qRT-PCR experiments showed that hearts at 14 dpci exposed to stress or dexamethasone/adrenaline treatment for 1 h d^-1 display a decrease in igfbp1b expression (N ≥ 3 sets of 10 cryoinjured ventricles). ***p < 0.001, ****p < 0.0001. Dashed lines encircle the cryoinjured areas. Scale bars, 100 µm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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