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Morpholino-induced knockdown of YY1a results in down-regulation of PS receptor at 24 hpf. a Western-blot analysis of PSR proteins in lysates of YY1-MO-injected embryos. Note that PSR protein level in these embryos decreases in a YY1-MO dose-dependent manner (Fig. 5a, panel a, lane 1 [control-MO, 50 ng]; lane 2 [YY1-MO, 25 ng], and lane 3 [YY1-MO, 50 ng]). Panel b shows the actin loading control. b One-cell stage embryos were each injected with either 25 ng of control-MO or 25 ng of YY1-MO. At 12, 24, and 48 hpf, embryos were fixed and in-situ hybridization was performed as described in the Methods section. Phase-contrast images show normal development and psr expression patterns in wild type (panels a, d, and g) and control-MO-injected (panels b, e, and h) embryos but abnormal development (panels c, f, and i) and either a mild (panel c, 12 hpf) or severe (panel f, 24 hpf; panel i, 48 hpf) delay in psr expression (arrows) in YY1-MO-injected embryos. Bars indicate 100 µm
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