FIGURE

Fig. 3

ID

ZDB-FIG-160217-2

Publication

Ge et al., 2014 - Hecate/Grip2a acts to reorganize the cytoskeleton in the symmetry-breaking event of embryonic axis induction

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Fig. 3

Whole mount in situ hybridization analysis of the expression of zebrafish grip2a mRNA in early embryos.

A-E) grip2a mRNA is localized in a slightly off-center position at the vegetal pole of early embryos, and remains in that position until the sphere stage when it becomes undetectable. F) Control sense probe. G) The domain of grip2a mRNA localization in hec mutants is reduced in intensity and appears to lack an off-center shift. H,I) Representative control (DMSO)- and nocodazole-treated embryos showing the lack of off-center shift in nocodazole-treated embryos (see Figure S4 for details). All panels are side views (except for (B), which is a vegetal view of the embryo in (A)) at the following stages: A,B: 1-cell (20 mpf), C,F,G: 2-cell (45 mpf), D: 64-cell (2 hpf), E: sphere (4 hpf). (H,I) are at 40 mpf, which approximately corresponds to the 2-cell stage (C) in untreated embryos. Magnification bar in (I) corresponds to 100 µm for all panels.

Expression Data

Gene:	grip2a
Fish:	WT grip2a^p06ucal grip2a^p08ajug grip2a^t2800
Condition:	chemical treatment: nocodazole
Anatomical Term:	yolk animal-vegetal axis
Stage Range:	1-cell to Sphere

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT grip2a^p06ucal grip2a^p08ajug grip2a^t2800
Condition:	chemical treatment: nocodazole
Observed In:	yolk animal-vegetal axis
Stage:	2-cell

Phenotype Detail

Acknowledgments

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