Fig. 1
- ID
- ZDB-FIG-151214-35
- Publication
- Manning et al., 2015 - Tbx16 and Msgn1 are required to establish directional cell migration of zebrafish mesodermal progenitors
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Tbx16 and Msgn1 act cell-autonomously in migration out of the tailbud. (A) Diagram of transplant scheme. Labelled undifferentiated cells are removed from donor embryos and placed in the ventral margin (fated to become tail somites) of unlabeled gastrulating embryos. B, C and E, F. Fluorescently labelled donor cells (red) overlaid on bright field images of host embryos at 24 h post-fertilization. (B) Wild-type donor cells in wild-type host. (C) MO donor cells in wild-type host. (E) Wild-type donor cells in MO host. (F) MO donor cells in MO host. Brackets and arrow indicate locations of donor cells. (D) Percentage of wild-type host embryos containing donor cells in somite, fin or epithelium, and undifferentiated groups. *: p<0.01 by Χ2 test. (G) Percentage of MO host A–P body length containing donor cells. (Distance from posterior of embryo to anterior-most donor cell divided by total A-P body length.) *: p<0.01 by Anova. Bars show standard deviation. (H) 24 h post-fertilization MO host with somite-like organization of wild-type donor cells. Left panel is a single frame bright field image; middle is a Z projection through 4 µm of fluorescent dextran-labeled donor cells; right is a Z projection through 4 µm of embryos stained with a muscle myosin antibody. Arrows show somite-like structures formed from donor cells. Scale bar is 50 µm. |
Reprinted from Developmental Biology, 406(2), Manning, A.J., Kimelman, D., Tbx16 and Msgn1 are required to establish directional cell migration of zebrafish mesodermal progenitors, 172-85, Copyright (2015) with permission from Elsevier. Full text @ Dev. Biol.