FIGURE

Fig. 4

ID

ZDB-FIG-151110-32

Publication

Radev et al., 2015 - A TALEN-Exon Skipping Design for a Bethlem Myopathy Model in Zebrafish

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Fig. 4

Validation of the presence of col6a1 mRNA by RT-PCR at different developmental stages in different organs of zebrafish.

(A) Confirmation of the presence of col6a1 mRNA in different organs of adult WT zebrafish: col6a1 mRNA is detected in the electrophoregram of organs from adult fish as indicated at the top of the panel. β-actin is used as internal control. (B) Confirmation of the presence of two forms of col6a1 mRNA in wild-type (WT) and mutant (presenting exon 14 skipping) zebrafish embryos at 2 dpf from col6a1^ama605003 line. WT fish express only the wild type allele (298 bp); heterozyogous mutant (HT) carry both wild type and mutated allele (244 bp) and homozygous fish (HM) express only the exon-skipped form.

Expression Data

Gene:	col6a1
Fish:	TU
Anatomical Terms:	anatomical structure caudal fin intestine mandibular arch skeleton muscle spleen
Stage Range:	Long-pec to Adult

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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