Fig. 4

The circadian expression of three impdh homologs show robust in larvae and adult zebrafish tissues.

The expression of impdh1a (A) is not circadian oscillating in adult zebrafish brain (red line) but shows circadian rhythm in 5dpf larval whole body (black line). impdh1b (B) and impdh2 (C) are circadian oscillating with circadian phases around CT12 and CT23 respectively in both adult zebrafish brain and larvae. The solid curves represent microarray data and the dashed curves were fitted by cosine functions. (D) impdh1a is predominantly expressed in the adult eye where the expression level at ZT0 is much higher than that at ZT12. (E) impdh1b shows rhythmic expression in the brain, eye, heart and muscle. (F) Although the expression of impdh2 in the heart is higher than in other tissues, it shows the most significant expression change between ZT0 and ZT12 in the liver. Error bars represent the standard error of mean (SEM) among independent biological replicates. The gene expression levels at ZT0 and ZT12 were compared using a Student’s t-test (unpaired, two tailed; *** p<0.001; ** p<0.01; * p<0.05).