FIGURE

Fig. 2

ID

ZDB-FIG-150716-9

Publication

Rau et al., 2015 - Abnormal splicing switch of DMD's penultimate exon compromises muscle fibre maintenance in myotonic dystrophy

Other Figures

Fig. 2

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Fig. 2

Exclusion of dmd exon 78 in zebrafish impairs skeletal muscle development.

(a) RT–PCR of dmd exon 78 performed on total RNA extracts isolated from whole control and dmd Δ78 embryos (48 hpf). (b) Dose-dependant phenotype of dmd Δ78 embryos: control embryos (CTL) compared with moderate and severe affected dmd Δ78 morphants at 48 hpf (scale bar, 1 mm). (c) Touch-evoked escape test of control embryos compared with moderate and severe dmd Δ78 morphants (1 image/0.2 s; scale bar, 1 mm). (d) Abnormal myoseptum U-shape in dmd Δ78 morphants compared with V-shape in control embryos at 48 hpf (scale bar, 250 and 100 µm). (e) Dystrophin immunostaining (MANDRA1 antibody) of control embryos compared with dmd Δ78 morphants at 48 hpf. (f) Slow myosin immunostaining of control embryos compared with moderate and severe affected dmd Δ78 morphants at 48 hpf (scale bar, 50 and 10 µm).

Expression Data

Gene:	dmd
Antibody:	Ab1-dmd
Fish:	WT WT + MO7-dmd
Knockdown Reagent:	MO7-dmd
Anatomical Term:	vertical myoseptum
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO7-dmd
Knockdown Reagent:	MO7-dmd
Observed In:	post-vent region skeletal muscle cell myofibril skeletal muscle tissue development slow muscle cell startle response swimming behavior thigmotaxis vertical myoseptum
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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