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Fig. S1

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ZDB-FIG-150713-9

Publication

Wong et al., 2015 - Mutual antagonism of the paired-type homeobox genes, vsx2 and dmbx1, regulates retinal progenitor cell cycle exit upstream of ccnd1 expression

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Fig. S1

Over expression of vsx2 and knock down of vsx2 in wild type embryos. Embryos were injected with pSGH:vsx2, heat shocked at 24 hpf and fixed at 48 hpf. Cryosections of 20 µm were labeled with anti-vsx2 and anti-GFP (A–D) (n=5). Transcript levels of vsx2 were also measured using RT-qPCR (D). There was a significant increase in vsx2 gene expression at 72 hpf (D). Three pools of RNA from 50 embryos were collected for RT-qPCR. Standard deviation was calculated from three replicate experiments. Translational blocking morpholinos were injected into wild type embryos and these morphants displayed microphthalmia with a significant decrease in eye diameter (F–H). Western blot of protein from the translational blocking morpholino injected embryos displayed lower vsx2 protein (I). Standard deviation was calculated from five replicate experiments. Statistical significance was assessed using Student′s t-test (p<0.05).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 402(2), Wong, L., Power, N., Miles, A., Tropepe, V., Mutual antagonism of the paired-type homeobox genes, vsx2 and dmbx1, regulates retinal progenitor cell cycle exit upstream of ccnd1 expression, 216-28, Copyright (2015) with permission from Elsevier. Full text @ Dev. Biol.