FIGURE

Fig. 1

ID

ZDB-FIG-150710-8

Publication

Wang et al., 2015 - Fate mapping of ptf1a-expressing cells during pancreatic organogenesis and regeneration in zebrafish

Other Figures

All Figure Page

Back to All Figure Page

Fig. 1

Generation of the ptf1a lineage tracing system and its initial characterization. A: Schematic diagram of ptf1a:creER^T2 driver and responder ubi:loxP-CFP-loxP-nuc-mCherry. Double transgenic fish larvae were treated with 5 µM 4-OHT in E3 medium at 1 dpf for 24 hr and then fixed for imaging at 5 dpf. These larvae were raised along with untreated controls. B: At 5 dpf, no pancreatic and hindbrain nuclear-mCherry signals were detected in control larvae (a1–e1, f1–h1). 4-OHT-treated larvae showed nuclear-mCherry signal (red) in exocrine pancreas (a2–e2) and hindbrain (f2–h2), indicating ptf1a-dependent Cre activity. CFP signal (cyan) could be detected ubiquitously (c1, f1, c2, f2). DAPI (blue) stains for nuclei (b1, b2).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Dev. Dyn.