FIGURE

Fig. 3

ID

ZDB-FIG-150603-2

Publication

Tarbashevich et al., 2015 - Chemokine-Dependent pH Elevation at the Cell Front Sustains Polarity in Directionally Migrating Zebrafish Germ Cells

Other Figures

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Fig. 3

ca15b Knockdown Impairs PGC Migration

(A) Graph shows the percentage of ectopic PGCs from total PGC number in wild-type embryos at 24 hpf.

(B) Graph presents the average percentage of ectopic PGCs in 24 hpf embryos heterozygous for a mutation in cxcl12 (medusa mutation). Right panels show representative images of the PGC positioning in control and ca15b morphant embryos in wild-type (A) and in medusa^+/- embryos (B).

N is number of embryos analyzed. White boxes depict the normal position of PGCs in 24 hpf embryos, arrowheads point at PGCs located in ectopic positions. p < 0.05, p < 0.001 as determined by the Student’s t test. Error bars depict SEM.

Expression Data

Gene:	EGFP
Fish:	er1Tg er1Tg + MO1-ca15b cxcl12a^t30516/+; er1Tg cxcl12a^t30516/+; er1Tg + MO1-ca15b
Knockdown Reagent:	MO1-ca15b
Anatomical Term:	primordial germ cell
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	er1Tg + MO1-ca15b cxcl12a^t30516/+; er1Tg + MO1-ca15b
Knockdown Reagent:	MO1-ca15b
Observed In:	germ cell migration primordial germ cell
Stage:	Prim-5

Phenotype Detail

Acknowledgments

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