FIGURE

Fig. 6

ID

ZDB-FIG-150601-6

Publication

Gutierrez-Triana et al., 2014 - A vertebrate-conserved cis -regulatory module for targeted expression in the main hypothalamic regulatory region for the stress response

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Fig. 6

Nitroreductase-expressing cells inTg(otpECR6-E1b:nfsb-GFP)larvae display a reduction in the number of projections to the pituitary and an increase in apoptotic bodies when exposed to Mtz. A, TUNEL staining of the preoptic GFP expression domain in an untreated larva. B, TUNEL staining of the preoptic GFP expression domain in an Mtz-treated larva. C, Pituitary bundles of GFP-stained fibers in a control larva (arrow). D, The pituitary location in an Mtz-treated larva (arrow). Note that in the control larva, the GFP fibers reach the pituitary and no apoptosis is detected, but in the Mtz-treated larva, the pituitary innervation is disrupted and apoptosis is apparent in preoptic cell bodies. Scale bars: 50 µm.

Expression Data

Gene:	GFP
Fish:	hd14Tg
Condition:	chemical treatment: metronidazole
Anatomical Terms:	preoptic area preoptic area axon
Stage:	Day 6

Expression Detail

Gene	Fish	Conditions	Stage	Qualifier	Anatomy	Assay
GFP	hd14Tg	control	Day 6		preoptic area	IHC
GFP	hd14Tg	control	Day 6		preoptic area axon	IHC
GFP	hd14Tg	chemical treatment: metronidazole	Day 6	Not Detected	preoptic area	IHC
GFP	hd14Tg	chemical treatment: metronidazole	Day 6	Not Detected	preoptic area axon	IHC

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Antibody Labeling

No data available

Phenotype Data

Fish:	hd14Tg
Condition:	chemical treatment: metronidazole
Observed In:	preoptic area axon preoptic area neuron
Stage:	Day 6

Phenotype Detail

Acknowledgments

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