Spontaneous synaptic AMPAR currents obtained from M-cells. (A) Uninjected embryos (n = 7), control morpholino injected embryos (n = 8), translation-blocking morpholino injected embryos (n = 7), and splice-blocking morpholino injected embryos (n = 6) exhibit AMPAR currents at similar frequencies. The inclusion of NBQX (0.1 µM) confirmed the mEPSCs as AMPAR-mediated. Preparations were incubated in TTX (1 µm), strychnine (5 µM), picrotoxin (100 µM), and APV (50 µM) to block action potentials, glycine, GABA and NMDA receptors, respectively. Average traces are shown to the right of each recording; each trace is an average of multiple events; 215 mEPSCs for the uninjected controls, 224 events for the MO controls, 174 events for the translation-blocking morphants, and 276 events for the splice-blocking morphants. Bar graphs comparing the peak amplitude (B), frequency (C), 20–80% rise time (D), and decay time course (E) of averaged AMPA mEPSCs. * Significantly different from uninjected controls, p < 0.05.
|
