Fig. 2
miR-107 Posttranscriptionally Regulates Dicer during Neural Development (A–D) Lateral view of 24 hpf zebrafish embryos treated as indicated. Anterior is to the left. Black arrows indicate MHB in (A), (C), and (D). (E) Percentage of embryos without MHB observed in (A–D). Data are presented as mean ± SEM of five independent experiments (n ~20 embryos each), p < 0.05. (F) Northern Blot showing miR-430 biogenesis in 24 hpf embryos treated as indicated. WT (uninjected embryos) and miR-107-duplex-MUT were used as controls. The image is representative of two independent experiments. (G) qRT-PCR of miR-430 levels in 24 hpf embryos treated as indicated. Levels are relative to the WT control. Data are mean ± SEM (n = 4). p < 0.05. (H) Schematic representation of the miR-107 sensor assay shown in (I–J). (I) Fluorescent images of whole-mount zebrafish embryos at 24 hpf treated as indicated. Anterior is to the left. (J) Quantification of GFP and mCherry fluorescence levels in each experimental condition measured as fluorescence intensity per area. Data are presented as mean ± SD of three independent experiments (n = 5 to 10 embryos each). p < 0.05. Scale bars represent 50 µm. OV, otic vesicle. |
Reprinted from Developmental Cell, 32(5), Ristori, E., Lopez-Ramirez, M.A., Narayanan, A., Hill-Teran, G., Moro, A., Calvo, C.F., Thomas, J.L., Nicoli, S., A Dicer-miR-107 Interaction Regulates Biogenesis of Specific miRNAs Crucial for Neurogenesis, 546-60, Copyright (2015) with permission from Elsevier. Full text @ Dev. Cell