FIGURE

Fig. 7

ID

ZDB-FIG-150413-46

Publication

Jackson et al., 2015 - The role of Sox6 in zebrafish muscle fiber type specification

Other Figures

All Figure Page

Back to All Figure Page

Fig. 7

Sox6 controls the Ca2+ response of fast-twitch fibers. (A,B) Cross section through a trunk somite of a 48 hours postfertilization (hpf) Tg(smyhc1:GCaMP3)ⁱ²⁸⁰ embryo, showing expression of GCaMP3 (green) is restricted to slow-twitch fibers, revealed by mAbF59 labeling (red). Nuclei are stained with DAPI (blue). (C) Stereotypical representation of the GCaMP3 signal in response to chemical stimulation indicating the three parameters measured in this analysis. (D) Response time in seconds (s) and (E) amplitude of Ca2+ response in fast and slow-twitch fibers of Tg(smyhc1:GCaMP3)ⁱ²⁸⁰ (Tg) and transient transgenic wild-type (+) and sox6 mutant larvae (52 to 55 hpf); Student’s two-tailed, unpaired t-test; Error bars represent standard deviation.

Expression Data

Gene:	GCaMP
Antibody:	Ab-F59
Fish:	i280Tg
Anatomical Term:	slow muscle cell
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	sox6^i291/i291; i280Tg
Observed In:	fast muscle cell calcium-mediated signaling
Stage:	Prim-15

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Skelet Muscle