Fig. 1

Summary of genes with significantly altered expression in igu mutants and cyclopamine-treated embryos. A–C: Phenotypes of wild-type (A), igu mutant (B) and cyclopamine-treated wild-type (C) embryos at 48 hpf. Arrows point to hemorrhages. Scale bar = 100 µm. D: Venn diagram of total genes significantly up- or down-regulated in treatment group as compared to their control. The purple circle represents the comparison of igu mutants and wild-type siblings and the blue circle represents the comparison of cyclopamine-treated to DMSO-treated siblings. The overlap represents 40 annotated genes that were changed in both groups. E: Heat map of 40 genes differently expressed in both igu mutant and cyclopamine-treated embryos. Green indicates down-regulation, and red indicates up-regulation. Values on scale bar indicate range of fold changes from 6.2-fold down-regulated to 2.1-fold up-regulated. igu, iguana mutant; cyc, cyclopamine-treated.