FIGURE

Fig. 4

ID

ZDB-FIG-150402-26

Publication

Seredick et al., 2014 - Lhx3 and Lhx4 suppress Kolmer-Agduhr interneuron characteristics within zebrafish axial motoneurons

Other Figures

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Fig. 4

Islet and Lhx3/4 proteins are independently regulated in ventral spinal cord. (A-F) Lateral views of 28hpf embryos. MN Islet expression is absent from islet1 MO-injected embryos (A,B), but persists in lhx3+lhx4 MO-injected embryos (C). Lhx3/4 expression persists in islet1 MO-injected embryos (D,E) and is absent from lhx3+lhx4 MO-injected embryos (F). (G) Quantification of Islet⁺ PMNs and early-born SMNs in presence or absence of Lhx3 and Lhx4 (P=0.18; control, n=56 spinal hemisegments in seven embryos; MOs, n=80 spinal hemisegments in ten embryos). (H,I) Sections of ventral spinal cord of 48hpf embryos labeled with anti-Islet. Islet expression persists in embryos injected with lhx3+lhx4 MOs. (J) Quantification of Islet⁺ MNs in the presence or absence of Lhx3 and Lhx4 (P=0.44; n=10 alternating sections from ten embryos for both conditions). Scale bars: 20µm.

Expression Data

Antibodies:	ab1-lhx3/4 Ab2-isl
Fish:	WT WT + MO2-isl1a + MO3-isl1a WT + MO1-lhx3 + MO1-lhx4
Knockdown Reagents:	MO1-lhx3 MO1-lhx4 MO2-isl1a MO3-isl1a
Anatomical Terms:	primary motor neuron cell body secondary motor neuron cell body
Stage Range:	Prim-5 to Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-lhx3 + MO1-lhx4
Knockdown Reagents:	MO1-lhx3 MO1-lhx4
Observed In:	primary motor neuron secondary motor neuron spinal cord
Stage Range:	Prim-5 to Long-pec

Phenotype Detail

Acknowledgments

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